Biothreat Detection

Real confirmation of potentially deadly samples

Brukers pBDi uses a sandwich electrochemical ELISA procedure for the highly sensitive and fully automated detection of all kinds of biothreat agents (bacteria, viruses and toxins). Antibodies immobilized on gold electrodes attached to electrodes of a biochip facilitate the specific capture of corresponding biothreat agents from an applied sample.brukers pbdi

The detection of the captive biothreat agents is realized by measuring the electrical current of an enzymatic redox reaction.The current correlates to the amount of target bound to the specific antibodies. Up to 6 biothreat agents can be rapidly detected in parallel within 20 minutes assay time.

Portable Bio Detector

Biological Threat Mitigation

The Bruker pBDi is a portable detection platform for rapid and sensitive on-site identification of biothreat agents. Developed for use by non-scientific personnel, the pBDi is easily operated, even while working in protective equipment under extreme conditions.

Fully portable and operating from internal batteries, pBDi can be used in the hot zone. Equally pBDi can be integrated with various mobile platform solutions, where it can be powered from an external supply. The pBDi employs a sensitive electrochemical biochip technology for multiplex ELISA-based (enzymelinked immunosorbent assay) detection of biothreat agents.

The pBDi builds on the technology of the Bruker portable Toxin Detector and offers new features such as integration with a mobile suitcase, battery operation, Bluetooth connection to a ruggedized tablet PC and assays for bacteria, viruses and toxins identification.

The detection principle of the pBDi is based on the well established ELISA procedure combined with an electrochemical readout.

Capture antibodies immobilized on gold electrodes facilitate the specific binding of corresponding biothreat agents. Detection of bound biothreat agents is realized by application of a detector-antibody-enzyme conjugate and measurement of the electrical current of an enzymatic redox reaction.

The electric signal is strongly amplified in this system and allows very sensitive biothreat agent detection in approx. 20 minutes. First, the high turnover of enzymatic reaction contributes to the signal amplification and second, a redox cycling procedure built into the experimental procedure, provides an additional signal amplification. The straightforward workflow starts with resuspension of a liquid or solid sample in a supplied sample buffer.

pBDi Features

  • Broad application range: Universal and reliable detection of up to 6 toxins, bacteria and viruses in parallel assayed in duplicates along with internal assay controls
  • Easy-to-use ELISA-based technology:Special cartridge and reagent holder design enable safe handling by unskilled operators.
  •  Automated sample processing and data evaluation: Wizard-based control software with fully-automated data processing and “traffic light” based result display.

Principle of electrochemical immunoassay

Principle of electrochemical immunoassay

  • Outstanding test time combined with high sensitivity: Receive test results in about 20 mins with sensitivities down to pg/ml range for toxins; 10³ CFU/ml for bacteria and 104PFU/ml for viruses.
  •  Portability and possibility to decontaminate: Battery-based operation modus and suitcase integration enable field and mobile applications by first responders.
  •  Freeze dried ready-to-use kits: Enable long-time storage up to 12 months and reduce ownership costs.
  •  Open array concept: Self-immobilization protocols enabling users to create their own assays.

Connecting your Detectors with CBRNetTM

CBRNet™ is a networked system in which sensor instruments are digitally linked to your key decision makers and to professional CBRNe advisers. Decision makers will have access to a real time Recognised CBRNe Picture of your operation, showing the location of your sensors, the data they are collecting and the way they are performing, all supporting the ability to make quick, accurate decisions.

The data from CBRNe sensors is brought into SafeZone via the Internet, thus allowing you to access it wherever you need. At all stages it is fully protected so that it guarantees high confidence levels in measures of availability, integrity, authentication, confidentiality and non- repudiation.

CBRNet™ enables the exchange of data between your operations rooms and deployed CBRNe sensors, using the dedicated information system, SafeZone. At the front-end, data collected by your instrument detectors passes over whatever tactical communications system you choose. The networking technology allows for the use of mobile telephony, terrestrial and satellite radios and can harness the power of Bluetooth and WiFi connections. These advanced communication technologies are provided by Cubic Mission Solutions, world leaders in the industry and widely used by NATO special forces and government agencies.

Central Monitoring, Advice & Global Incident Capability

CBRNe expert support is provided by CBRNet™ staff from our Headquarters in Leipzig, Germany, and Billerica, USA, world centres of scientific excellence. The CMAGIC™ operational facilities (OF) provide constant cover with access to scientific and technical advice, as well as training and fleet management expertise.

CBRNet™ utilises an open, Service Oriented Architecture (SOA) in order to ensure that you can, if you wish, easily interface its output to your existing command systems.